Stable Cell Line Development

The jump in technology helps accelerate stable cell line development due to the targeted integration of genetic material into specific integration sites using phic31 integrase.

Stable cell line development.

Traceability of cell history and cell line generation process high productivity and robust stable cell line development platforms. Stable cell line generation. This protocol describes the procedure for creating a stable cell line based on the expicho expression system for use in commercial bioproduction. Gpex cell line development technology is a proven technology that generates high performance highly stable production cell lines.

By applying single cell clonings from the selected stable pools selexis identifies high expressing clonal cell lines that are then evaluated for their. We understand that cell line development involves multiple complex processes requiring consistency and reproducible results. One is achieved by eukaryotic vectors that harbor elements for episomal maintenance in the nucleus of a transfected cell. That s why gibco products are designed to provide you with integrated stable cell line development solutions to simplify your development workflow and accelerate your processes to commercial manufacture.

Expicho s cells are transfected cloned and selected in expicho expression medium followed by the scale up in the bioproduction amenable expicho stable production medium. Stable expression of target gene in cell lines overcomes the low transfection efficiency of transient expression and produces more target proteins. These optimally designed dna expression vectors are then transfected into the fully documented selexis sure cho m cell line using the sure fection procedure. Typical cell line workflow step 1.

Proteogenix strives to overcome the economic issues related to stable cell line generation by offering the most productive ip free cell lines on the market. There are two types of stable cell lines. After several passages some stable cell lines may become unstable with their expression diminishing slowly. Foreign dna encoding the protein of interest often includes an additional selectable marker.

However not all stable cell lines are equally stable. You want to develop a stable cell line because it is stable in expressing the target protein. Virtually any cdna mab and multigenic applications can be packaged into the gpex retrovector and used to insert your gene of interest into cells. The process of cell line development begins with the introduction of foreign dna encoding.

This is a very common problem for many stable cell lines. The development of high yield production cell lines starts with the cloning of the gene of interest into sure tech vectors that bear selexis sge selexis genetic elements.